SSX2IP antibody (AA 362-411)

Catalog No. ABIN6741616

This anti-SSX2IP antibody is a Rabbit Polyclonal antibody detecting SSX2IP in WB, IHC and IHC (p). Suitable for Human, Dog, Cow, Guinea Pig, Horse, Rabbit, Monkey and Pig.

Quick Overview for SSX2IP antibody (AA 362-411) (ABIN6741616)

Target: SSX2IP (Synovial Sarcoma, X Breakpoint 2 Interacting Protein (SSX2IP))

Reactivity: Human, Dog, Cow, Guinea Pig, Horse, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSX2IP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SSX2IP Antibody

Binding Specificity: AA 362-411

Brand: IHC-plus™

Specificity: Human SSX2IP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Mouse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa362-411 of human SSX2IP (Q9Y2D8, NP_054740). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Platypus (100%), Galago, Mouse, Rat, Bat, Opossum (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SSX2IP

Target: SSX2IP (Synovial Sarcoma, X Breakpoint 2 Interacting Protein (SSX2IP))

Alternative Name: SSX2IP

Background: Name/Gene ID: SSX2IP

Synonyms: SSX2IP, ADIP, KIAA0923, SSX2-interacting protein

Gene ID: 117178

NCBI Accession: NP_054740