ERF antibody (N-Term)

Catalog No. ABIN6742012

This anti-ERF antibody is a Rabbit Polyclonal antibody detecting ERF in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Bat, Monkey and Xenopus laevis.

Quick Overview for ERF antibody (N-Term) (ABIN6742012)

Target: ERF (Ets2 Repressor Factor (ERF))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Bat, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERF Antibody

Binding Specificity: N-Term

Specificity: Human ERF / PE-2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human ERF (P50548, NP_006485). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Platypus, Xenopus, Zebrafish (100%), Tamarin, Hamster, Opossum, Sea squirt (92%), Zebra finch (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ERF

Target: ERF (Ets2 Repressor Factor (ERF))

Alternative Name: ERF / PE2

Background: Name/Gene ID: ERF

Synonyms: ERF, PE-2, Ets2 repressor factor, PE2

Gene ID: 2077

NCBI Accession: NP_006485

UniProt: P50548