MELK antibody (AA 432-481)

Catalog No. ABIN6743416

This Rabbit Polyclonal antibody specifically detects MELK in WB. It exhibits reactivity toward Human, Cow, Horse, Guinea Pig and Pig.

Quick Overview for MELK antibody (AA 432-481) (ABIN6743416)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity: Human, Cow, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MELK Antibody

Binding Specificity: AA 432-481

Specificity: Human MELK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse (100%) Dog, Rabbit (92%) Guinea pig (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa432-481 of human MELK (Q14680, NP_055606). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Horse (100%), Marmoset, Dog, Rabbit (92%), Monkey, Elephant, Bat (85%), Guinea pig (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK

Background: Name/Gene ID: MELK
Subfamily: CAMKL
Family: Protein Kinase

Synonyms: MELK, KIAA0175, HPK38, Protein kinase Eg3, Tyrosine-protein kinase MELK, Protein kinase PK38, HMELK, Mpk38, PEg3 kinase

Gene ID: 9833

NCBI Accession: NP_055606

UniProt: Q14680