MYL6 antibody (AA 72-121)

Catalog No. ABIN6744460

This anti-MYL6 antibody is a Rabbit Polyclonal antibody detecting MYL6 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit, Pig, Zebrafish (Danio rerio), Bat and Monkey.

Quick Overview for MYL6 antibody (AA 72-121) (ABIN6744460)

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit, Pig, Zebrafish (Danio rerio), Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MYL6 Antibody

Binding Specificity: AA 72-121

Brand: IHC-plus™

Specificity: Human MYL6

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human MYL6 (P60660, NP_524147). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Lizard (100%), Gibbon, Panda, Opossum (91%), Platypus (90%), Hamster, Turkey, Zebra finch, Chicken, Zebrafish (85%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MYL6

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Alternative Name: MYL6

Background: Name/Gene ID: MYL6

Synonyms: MYL6, 17 kDa myosin light chain, ESMLC, LC17, LC17B, LC17-GI, LC17A, MLC3NM, MLC1SM, MLC3SM, Myosin light chain A3, Myosin light chain 3, LC17-NM, MLC-3, Myosin light polypeptide 6, Myosin light chain alkali 3

Gene ID: 4637

NCBI Accession: NP_524147

UniProt: P60660