NFKBIA antibody (AA 1-120)

Catalog No. ABIN674454

Product Details anti-NFKBIA Antibody

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Binding Specificity: AA 1-120

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Chicken, Cow, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse IKB alpha

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NFKBIA antibody (ABIN674454)

Yang, Cheng, Liu, Liu, Tan, Guan, Guo, Kuang: "Datura Metel L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8-MyD88-NF-κB-NLRP3 Inflammasome Pathway." in: Molecules (Basel, Switzerland), Vol. 24, Issue 11, (2019) (PubMed).

Wang, Liu, Wang, Wang, Tang, Jiang: "Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-κB Signaling." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 1282-1294, (2018) (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 192-200, (2017) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Zhu, Jiang, Li, Zhao, Li: "S-Allylmercaptocysteine Attenuates  Cisplatin-Induced Nephrotoxicity through  Suppression of Apoptosis, Oxidative Stress, and  Inflammation." in: Nutrients, Vol. 9, Issue 2, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).

Gao, Guo, Wang, Lin, Yu, Sheng, Wu, Gong: "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." in: Acta histochemica, Vol. 118, Issue 6, pp. 606-14, (2016) (PubMed).

Zhao, Zheng, Hu, Shen, Li: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." in: Life sciences, Vol. 144, pp. 185-93, (2016) (PubMed).

Li, Zhang, Qi, Zhang, Yi, Li, Zhang: "Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB." in: The journal of headache and pain, Vol. 17, pp. 49, (2016) (PubMed).

Yu, Shi, Qi, Zhao, Gao, Li: "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." in: Frontiers in pharmacology, Vol. 7, pp. 148, (2016) (PubMed).

Han, Xie, Sui, Liu, Du, Liu, Feng, Jiang: "Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects." in: Molecular medicine reports, Vol. 14, Issue 3, pp. 2589-97, (2016) (PubMed).

Zhang, Ma, Zhang, Dai, Chang, Huang, Tian: "Hyperbaric Oxygen and Ginkgo Biloba Extract Ameliorate Cognitive and Memory Impairment via Nuclear Factor Kappa-B Pathway in Rat Model of Alzheimer's Disease." in: Chinese medical journal, Vol. 128, Issue 22, pp. 3088-93, (2016) (PubMed).

Tang, Lv, Sun, Han, Zhou: "14-3-3β Promotes Migration and Invasion of Human Hepatocellular Carcinoma Cells by Modulating Expression of MMP2 and MMP9 through PI3K/Akt/NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146070, (2016) (PubMed).

Chen, Zhang, Ma, Huang, Shao, Wu, Wang: "Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143868, (2016) (PubMed).

Yan, Wu, Li, Meng, Dai, Zheng, Fu: "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-?-Treated Rat Aortic Smooth Muscle Cells." in: Cardiovascular toxicology, (2015) (PubMed).

Ma, Liu, Wang, Liu, Chen, Valle, Zuo, Xia, Liu: "Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." in: ACS nano, Vol. 9, Issue 10, pp. 10498-515, (2015) (PubMed).

Target Details for NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Alternative Name: NFKBIA/IKB alpha (NFKBIA Products)

Background:

Synonyms: Nfkbi, AI46215, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Nfkbia, Ikba

Background: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Gene ID: 18035

UniProt: Q9Z1E3

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling