RHOJ antibody (AA 71-120)

Catalog No. ABIN6745148

The Rabbit Polyclonal anti-RHOJ antibody has been validated for WB. It is suitable to detect RHOJ in samples from Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat, Zebrafish (Danio rerio), Bat, Chicken, Monkey and Pig.

Quick Overview for RHOJ antibody (AA 71-120) (ABIN6745148)

Target: RHOJ (Ras Homolog Gene Family, Member J (RHOJ))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat, Zebrafish (Danio rerio), Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOJ antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RHOJ Antibody

Binding Specificity: AA 71-120

Specificity: Human RHOJ

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Chicken, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human RHOJ (Q9ER71, NP_065714). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Zebrafish (100%), Xenopus, Frog (92%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RHOJ

Target: RHOJ (Ras Homolog Gene Family, Member J (RHOJ))

Alternative Name: TCL / RHOJ

Background: Name/Gene ID: RHOJ
Family: Ras GTPase superfamily IPR001806

Synonyms: RHOJ, ARHJ, Ras homolog family member J, TC10-like Rho GTPase, TC10B, RAS-like, family 7, member B, RASL7B, RHOI, Tc10-like GTP-binding protein, TCL

Gene ID: 57381

NCBI Accession: NP_065714

UniProt: Q9H4E5