MED7 antibody (AA 143-192)

Catalog No. ABIN6745222

This Rabbit Polyclonal antibody specifically detects MED7 in WB. It exhibits reactivity toward Human, Mouse, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Pig, Bat, Hamster, Monkey, Chicken and Xenopus laevis.

Quick Overview for MED7 antibody (AA 143-192) (ABIN6745222)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Reactivity: Human, Mouse, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Pig, Bat, Hamster, Monkey, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED7 Antibody

Binding Specificity: AA 143-192

Specificity: Human MED7 / CRSP9

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Pig, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human MED7 (O43513, NP_004261). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Frog, Catfish, Salmon, Sablefish, Zebrafish (100%), Stickleback (92%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Alternative Name: MED7 / CRSP9

Background: Name/Gene ID: MED7

Synonyms: MED7, CRSP33, CRSP9, Mediator complex subunit 7, HMED7, ARC34, CRSP complex subunit 9

Gene ID: 9443

NCBI Accession: NP_004261

UniProt: O43513

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha