Nanog antibody (AA 135-184)

Catalog No. ABIN6746019

The Rabbit Polyclonal anti-Nanog antibody has been validated for WB. It is suitable to detect Nanog in samples from Human, Cow, Sheep and Monkey. There are 3+ publications available.

Quick Overview for Nanog antibody (AA 135-184) (ABIN6746019)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human, Cow, Sheep, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nanog Antibody

Binding Specificity: AA 135-184

Specificity: Human Nanog

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Pig (92%) Sheep, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa135-184 of human NANOG (Q6NSW7, NP_079141). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Dog, Cat, Horse, Pig, Opossum (92%), Sheep, Bovine, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-Nanog antibody (ABIN6746019)

Ku, Meier: "Phosphorylation of paxillin via the ERK mitogen-activated protein kinase cascade in EL4 thymoma cells." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11333-40, (2000) (PubMed).

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, (1999) (PubMed).

Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany, Sun, Pisick, Hallek, Ernst: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, (1995) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: Name/Gene ID: NANOG

Synonyms: NANOG, Nanog homeobox, NANOGP8, HNanog, Homeobox protein NANOG

Gene ID: 79923

NCBI Accession: NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance