PEX2 antibody (N-Term)

Catalog No. ABIN6746208

The Rabbit Polyclonal anti-PEX2 antibody has been validated for WB. It is suitable to detect PEX2 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Monkey, Pig and Hamster. There are 5+ publications available.

Quick Overview for PEX2 antibody (N-Term) (ABIN6746208)

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PEX2 Antibody

Binding Specificity: N-Term

Specificity: Mouse PEX2

Predicted Reactivity: Percent identity by BLAST analysis: Mouse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of mouse Pex2 (P55098, NP_033020). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Pig, Guinea pig (100%), Galago, Panda, Dog, Bat, Rabbit, Opossum, Lizard (92%), Zebra finch, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PEX2 antibody (ABIN6746208)

Kim, Lowenson, MacLaren, Clarke, Young: "Deficiency of a protein-repair enzyme results in the accumulation of altered proteins, retardation of growth, and fatal seizures in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6132-7, (1997) (PubMed).

MacLaren, Clarke: "Expression and purification of a human recombinant methyltransferase that repairs damaged proteins." in: Protein expression and purification, Vol. 6, Issue 1, pp. 99-108, (1995) (PubMed).

Galus, Lagos, Romanik, OConnor: "Structural analysis of transcripts for the protein L-isoaspartyl methyltransferase reveals multiple transcription initiation sites and a distinct pattern of expression in mouse testis: identification of a 5'-flanking sequence with promoter activity." in: Archives of biochemistry and biophysics, Vol. 312, Issue 2, pp. 524-33, (1994) (PubMed).

MacLaren, Kagan, Clarke: "Alternative splicing of the human isoaspartyl protein carboxyl methyltransferase RNA leads to the generation of a C-terminal -RDEL sequence in isozyme II." in: Biochemical and biophysical research communications, Vol. 185, Issue 1, pp. 277-83, (1992) (PubMed).

Romanik, Ladino, Killoy, DArdenne, OConnor: "Genomic organization and tissue expression of the murine gene encoding the protein beta-aspartate methyltransferase." in: Gene, Vol. 118, Issue 2, pp. 217-22, (1992) (PubMed).

Target Details for PEX2

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Alternative Name: PEX2 / PAF-1

Background: Name/Gene ID: PEX2

Synonyms: PEX2, PBD5B, PBD5A, Peroxisome assembly factor 1, Peroxisome assembly factor-1, PMP35, PXMP3, RNF72, PAF-1, ZWS3, Peroxin-2, Peroxisomal membrane protein 3, Peroxisome biogenesis factor 2, PMP3, RING finger protein 72

Gene ID: 5828

NCBI Accession: NP_033020

UniProt: P28328

Pathways: Monocarboxylic Acid Catabolic Process