Polybromo 1 antibody (AA 1511-1560)

Catalog No. ABIN6747037

The Rabbit Polyclonal anti-Polybromo 1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Polybromo 1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit, Bat, Chicken, Monkey and Pig.

Quick Overview for Polybromo 1 antibody (AA 1511-1560) (ABIN6747037)

Target: Polybromo 1 (PBRM1)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit, Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Polybromo 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Polybromo 1 Antibody

Binding Specificity: AA 1511-1560

Brand: IHC-plus™

Specificity: Human PBRM1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1511-1560 of human PBRM1 (Q86U86-4, NP_060783). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Lizard (92%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Polybromo 1

Target: Polybromo 1 (PBRM1)

Alternative Name: PBRM1 / BAF180 / PB1

Background: Name/Gene ID: PBRM1

Synonyms: PBRM1, BRG1-associated factor 180, HPB1, Polybromo 1, Protein polybromo-1, PB1, Polybromo-1D, BAF180

Gene ID: 55193

NCBI Accession: NP_060783

UniProt: Q86U86