beta Actin antibody (C-Term)

Catalog No. ABIN6747042

This Rabbit Polyclonal antibody specifically detects beta Actin in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Zebrafish (Danio rerio), Horse, Guinea Pig, Insect, Drosophila melanogaster and Xenopus laevis.

Quick Overview for beta Actin antibody (C-Term) (ABIN6747042)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Zebrafish (Danio rerio), Horse, Guinea Pig, Insect, Drosophila melanogaster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-beta Actin Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human ACTB / Beta-actin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ACTB (P60709, NP_001092). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Mouse, Rat, Sheep, Hamster, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus, Trout, Seabass, Salmon, Smelt, Medaka, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB / Beta Actin

Background: Name/Gene ID: ACTB

Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

Gene ID: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction