MAP4K4 antibody (AA 287-336)

Catalog No. ABIN6747124

The Rabbit Polyclonal anti-MAP4K4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect MAP4K4 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Pig, Bat, Chicken and Xenopus laevis.

Quick Overview for MAP4K4 antibody (AA 287-336) (ABIN6747124)

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Pig, Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAP4K4 Antibody

Binding Specificity: AA 287-336

Brand: IHC-plus™

Specificity: Human MAP4K4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa287-336 of human MAP4K4 (O95819, NP_004825). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%), Monkey, Platypus, Lizard, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAP4K4

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Alternative Name: MAP4K4 / NIK

Background: Name/Gene ID: MAP4K4
Subfamily: MSN
Family: Protein Kinase

Synonyms: MAP4K4, HGK, HPK/GCK-like kinase HGK, KIAA0687, Misshapen, NIK, Nck-interacting kinase, HPK/GCK-like kinase, Ste20 group protein kinase HGK, MEKKK 4, MEKKK4, Zc1, FLH21957, MEK kinase kinase 4

Gene ID: 9448

NCBI Accession: NP_004825

UniProt: O95819