Gelsolin antibody (AA 542-591)

Catalog No. ABIN6747152

The Rabbit Polyclonal anti-Gelsolin antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Gelsolin in samples from Human, Rat, Cow, Monkey, Horse, Sheep, Zebrafish (Danio rerio), Guinea Pig, Bat and Pig.

Quick Overview for Gelsolin antibody (AA 542-591) (ABIN6747152)

Target: Gelsolin (GSN)

Reactivity: Human, Rat, Cow, Monkey, Horse, Sheep, Zebrafish (Danio rerio), Guinea Pig, Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Gelsolin Antibody

Binding Specificity: AA 542-591

Brand: IHC-plus™

Specificity: Human GSN / Gelsolin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Bovine, Pig, Guinea pig, Zebrafish (100%) Mouse, Dog, Chicken (92%) Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa542-591 of human GSN (P06396, NP_000168). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Platypus, Zebrafish (100%), Gorilla, Galago, Mouse, Elephant, Dog, Turkey, Chicken, Stickleback (92%), Rat, Hamster (85%), Salmon (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: GSN / Gelsolin

Background: Name/Gene ID: GSN

Synonyms: GSN, ADF, AGEL, Brevin, Actin-depolymerizing factor, Gelsolin precursor, plasma, Gelsolin

Gene ID: 2934

NCBI Accession: NP_000168

UniProt: P06396

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy