GCLM antibody (AA 182-231)

Catalog No. ABIN6747158

The Rabbit Polyclonal anti-GCLM antibody has been validated for WB, IHC and IHC (p). It is suitable to detect GCLM in samples from Human, Mouse, Rat, Cow, Rabbit, Monkey, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Pig and Bat.

Quick Overview for GCLM antibody (AA 182-231) (ABIN6747158)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Monkey, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GCLM Antibody

Binding Specificity: AA 182-231

Brand: IHC-plus™

Specificity: Human GCLM

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (100%) Chicken (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa182-231 of human GCLM (P48507, NP_002052). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken (92%), Xenopus (85%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background: Name/Gene ID: GCLM

Synonyms: GCLM, Gamma-ECS regulatory subunit, GLCLR, GCS light chain, GSC light chain

Gene ID: 2730

NCBI Accession: NP_002052

UniProt: P48507