PDE4D antibody (AA 164-213)

Catalog No. ABIN6749764

The Rabbit Polyclonal anti-PDE4D antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PDE4D in samples from Human, Mouse, Rat, Horse, Chicken, Pig, Monkey and Xenopus laevis.

Quick Overview for PDE4D antibody (AA 164-213) (ABIN6749764)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human, Mouse, Rat, Horse, Chicken, Pig, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDE4D Antibody

Binding Specificity: AA 164-213

Brand: IHC-plus™

Specificity: Mouse PDE4D

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Horse, Pig (100%) Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa164-213 of mouse Pde4d (Q01063, NP_035186). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Horse, Pig, Zebra finch, Chicken, Lizard, Xenopus (100%), Rabbit, Opossum, Platypus (92%), Stickleback (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: PDE4D

Background: Name/Gene ID: PDE4D
Subfamily: PDE - cAMP-specific
Family: Phosphodiesterase

Synonyms: PDE4D, ACRDYS2, DPDE3, HSPDE4D, PDE43, Pde-ivd, PDE4DN2, Phosphodiesterase 4d, STRK1

Gene ID: 5144

NCBI Accession: NP_035186

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling