SFRP1 antibody (AA 31-313)

Catalog No. ABIN6757422

The Goat Polyclonal anti-SFRP1 antibody has been validated for WB and ELISA. It is suitable to detect SFRP1 in samples from Human.

Quick Overview for SFRP1 antibody (AA 31-313) (ABIN6757422)

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SFRP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SFRP1 Antibody

Binding Specificity: AA 31-313

Specificity: Recognizes human sFRP-1. Shows less than 1 % cross-reactivity with rmsFRP-1, rhsFRP-3 and rhsFRP-4.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rhsFRP-1 (aa31-313).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Direct ELISA and Western Blot. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human sFRP-1. The detection limit for rhsFRP-1 is ~0.6 ng/well. Testing was performed using the recombinant antigen as the sample. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human sFRP-1. The detection limit for rhsFRP-1 is ~5 ng/lane under non-reducing and reducing. conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: SFRP1

Background: Name/Gene ID: SFRP1
Subfamily: Frizzled

Synonyms: SFRP1, FrzA, FRP, SARP-2, SARP2, SFRP-1, FRP-1, FRP1

Gene ID: 6422

UniProt: Q8N474

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation