CCL8 antibody (AA 20-97)

Catalog No. ABIN676298

The Rabbit Polyclonal anti-CCL8 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) and FACS. It is suitable to detect CCL8 in samples from Mouse. There are 2+ publications available.

Quick Overview for CCL8 antibody (AA 20-97) (ABIN676298)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Product Details anti-CCL8 Antibody

Binding Specificity: AA 20-97

Cross-Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse MCP-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CCL8 antibody (ABIN676298)

Terao, Itoi, Matsumura, Yang, Murota, Katayama: "Local Glucocorticoid Activation by 11β-Hydroxysteroid Dehydrogenase 1 in Keratinocytes: The Role in Hapten-Induced Dermatitis." in: The American journal of pathology, Vol. 186, Issue 6, pp. 1499-510, (2016) (PubMed).

Nguyen, Fentress, Qiu, Yun, Cox, Chawla: "Circadian gene Bmal1 regulates diurnal oscillations of Ly6C(hi) inflammatory monocytes." in: Science (New York, N.Y.), Vol. 341, Issue 6153, pp. 1483-8, (2013) (PubMed).

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP-2/CCL8

Background:

Synonyms: HC14, MCP2, MCP-2, SCYA8, SCYA10, C-C motif chemokine 8, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, CCL8

Background: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

Gene ID: 6355

UniProt: P80075