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Cytokeratin 18 antibody (AA 53-150)

Name: Cytokeratin 18 antibody (AA 53-150)
SKU: ABIN676973
Price: 384.62 USD
Availability: InStock

KRT18 Reactivity: Human, Mouse, Rat, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded pig liver labeled with Anti-CK18 Polyclonal Antibody, Cy3 Conjugated (ABIN676973) at 1:200 for 60 minutes at 37C. (Cytokeratin 18 antibody (AA 53-150))

IHC

Immunofluorescence (IF) image for anti-Keratin 18 (KRT18) (AA 53-150) antibody (ABIN676973) (Cytokeratin 18 antibody (AA 53-150))

Formalin-fixed and paraffin embedded rat liver labeled with Anti-CK18 Polyclonal Antibody, Unconjugated (ABIN676973) at 1:800, followed by conjugation to the secondary antibody and DAB staining. (Cytokeratin 18 antibody (AA 53-150))

Rat Large intestine lysates probed with Cytokeratin 18 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (Cytokeratin 18 antibody (AA 53-150))

Paraformaldehyde-fixed, paraffin embedded human colon, Antigen retrieval by boiling in sodium citrate buffer (pH6. (Cytokeratin 18 antibody (AA 53-150))

IHC (p)

PC12 cells probed with Rabbit Anti-CK18 Polyclonal Antibody Alexa Fluor 488 Conjugated Secondary Antibody. (Cytokeratin 18 antibody (AA 53-150))

FACS

6 images

(4 references)

Catalog No. ABIN676973

Datasheet as PDF

Target See all Cytokeratin 18 (KRT18) Antibodies

Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
Binding Specificity
23

14

14

14

12

12

6

6

6

5

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 53-150
Reactivity
231

122

90

39

34

27

22

19

19

18

16

14

14

14

7

7

6

5

4

3

2

1
Human, Mouse, Rat, Pig
Host
138

119

3

1

1

1
Rabbit
Compatible Secondaries
Clonality
139

121

3
Polyclonal
Conjugate
142

19

18

10

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1
This Cytokeratin 18 antibody is un-conjugated
Application
208

97

84

73

61

54

50

36

36

31

22

19

7

7

6

5

2

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity

Human, Mouse, Pig, Rat

Predicted Reactivity

Dog,Cow,Horse,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CK18

Isotype

IgG
anti-Keratin 18 (KRT18) (AA 238-396) antibody
KRT18 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 1-423) antibody
KRT18 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 238-396) antibody
KRT18 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated

anti-Keratin 18 (KRT18) (AA 102-396) antibody
KRT18 Reactivity: Human WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 72-380) antibody
KRT18 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Keratin 18 (KRT18) (AA 401-430), (C-Term) antibody
KRT18 Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB18746 unconjugated

anti-Keratin 18 (KRT18) (AA 1-150) antibody
KRT18 Reactivity: Human WB, IHC, FACS, ELISA, ICC Host: Mouse Monoclonal 6G12F2 unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 Reactivity: Human WB, IHC, IF, FACS, StM Host: Mouse Monoclonal KRT18-834 unconjugated

anti-Keratin 18 (KRT18) antibody
KRT18 Reactivity: Human WB, IHC, IF, FACS, StM, ISt Host: Rabbit Monoclonal KRT18-2808R unconjugated Recombinant Antibody

anti-Keratin 18 (KRT18) antibody
KRT18 Reactivity: Human, Dog, Cat WB, IHC, IF, FACS, StM Host: Mouse Monoclonal DE-K18 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Fagundes, Fernandes, Paraense, de Farias-Junior, Teixeira, Alves-Junior, Pinheiro, Crespo-López, Maia, Lima: "Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 7323627, (2017) (PubMed).

Liu, Feng, Dong, Yang, Li, Chen, Zhang, Wang, Zhou, Zhao: "Administration of BMSCs with muscone in rats with gentamicin-induced AKI improves their therapeutic efficacy." in: PLoS ONE, Vol. 9, Issue 5, pp. e97123, (2014) (PubMed).

Zeng, Zhang, Li, Yang, Chen, Liu: "A new method to isolate and culture rat kupffer cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e70832, (2013) (PubMed).
Target

Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name

CK18 (KRT18 Products)

Background

Synonyms: K18, CYK18, Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, KRT18, PIG46
Background: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Gene ID

3875

UniProt

P05783

Pathways

Apoptosis, Caspase Cascade in Apoptosis