Caldesmon antibody (pSer789)

Catalog No. ABIN677333

The Rabbit Polyclonal anti-Caldesmon antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). It is suitable to detect Caldesmon in samples from Rat and Mouse. There is 1 publication available.

Quick Overview for Caldesmon antibody (pSer789) (ABIN677333)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Caldesmon Antibody

Binding Specificity: pSer789

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Caldesmon around the phosphorylation site of Ser789

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Caldesmon antibody (ABIN677333)

Prayitnaningsih, Sujuti, Effendi, Abdullah, Anandita, Yohana, Permatasari, Widodo: "Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats." in: International journal of ophthalmology, Vol. 9, Issue 11, pp. 1568-1577, (2016) (PubMed).

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: Caldesmon

Background:

Synonyms: Caldesmon S789, Caldesmon Ser789, p-Caldesmon S789, p-Caldesmon Ser789, p-Caldesmon Ser789, CAD, CALD 1, CALD1, CALD1_HUMAN, Caldesmon 1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, Caldesmon, Caldesmon1, CDM, H CAD, HCAD, L CAD, LCAD, MGC21352, NAG22.

Background: This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jul 2008].

Gene ID: 800

Pathways: Myometrial Relaxation and Contraction