FZD5 antibody (PE-Cy7)

Catalog No. ABIN681866

The Rabbit Polyclonal anti-FZD5 antibody has been validated for WB and FACS. It is suitable to detect FZD5 in samples from Human, Mouse, Rat, Cow, Dog and Pig.

Quick Overview for FZD5 antibody (PE-Cy7) (ABIN681866)

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD5 antibody is conjugated to PE-Cy7

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-FZD5 Antibody (PE-Cy7)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Frizzled 5

Isotype: IgG

Application Details

Application Notes: IF (p) (1:100-500)
Not yet tested in other applications. Optimal working dilutions must be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FZD5

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Alternative Name: Frizzled 5

Background:

Synonyms: C2orf31, Frizzled homolog 5, Frizzled-5, Frizzled5, Fz 5, Fz-5, Fz5, FZD 5, hFz5, FZD5, FZD5_HUMAN, FzE 5, FzE5, HFZ 5, Seven transmembrane receptor frizzled 5, Wnt receptor, hFz8, Fz-8, Frizzled-8, FZD8.

Background: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 5 protein is believed to be the receptor for the Wnt5A ligand. Frizzled 5 has been reported to be expressed in fetal kidney, fetal and adult liver, fetal lung, and adult pancreas. ESTs have been isolated from bone, liver/spleen, placenta, and prostate libraries. Frizzled 5 was cloned from a retina cDNA library. Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

Molecular Weight: 62 kDa

Gene ID: 7855, 8325

Pathways: WNT Signaling