SCNN1A antibody (AA 201-300)

Catalog No. ABIN682243

This Rabbit Polyclonal antibody specifically detects SCNN1A in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Rat, Human and Mouse and has been mentioned in 3+ publications.

Quick Overview for SCNN1A antibody (AA 201-300) (ABIN682243)

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCNN1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SCNN1A Antibody

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Alpha-ENaC

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SCNN1A antibody (ABIN682243)

Scortegagna, Berthon, Settas, Giannakou, Garcia, Li, James, Liddington, Vilches-Moure, Stratakis, Ronai: "The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion." in: JCI insight, Vol. 2, Issue 23, (2019) (PubMed).

Yamamoto, Hotta, Maeda, Kataoka, Maeda, Hamakawa, Sasaki, Yasui, Asai, Kimura: "Mineralocorticoid receptor stimulation induces urinary storage dysfunction via upregulation of epithelial sodium channel expression in the rat urinary bladder epithelium." in: Journal of pharmacological sciences, Vol. 130, Issue 4, pp. 219-25, (2016) (PubMed).

Target Details for SCNN1A

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Alternative Name: Alpha-ENaC

Background:

Synonyms: BESC2, ENaCa, SCNEA, SCNN1, ENaCalpha, Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNN1A

Background: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

Gene ID: 6337

UniProt: P37088