ERK1/2 antibody (pThr202, pTyr204)

Catalog No. ABIN682933

This Rabbit Polyclonal antibody specifically detects ERK1/2 in WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro) and ICC. It exhibits reactivity toward Human, Mouse, Rat and Cow and has been mentioned in 11+ publications.

Quick Overview for ERK1/2 antibody (pThr202, pTyr204) (ABIN682933)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-ERK1/2 Antibody

Binding Specificity: pThr202, pTyr204

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human p44/42 MAPK around the phosphorylation site of (Thr202/Tyr204)

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ERK1/2 antibody (ABIN682933)

Jeon, Han, Lee, Park, Kim, Yoo, Lee: "Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors." in: Scientific reports, Vol. 8, Issue 1, pp. 5415, (2019) (PubMed).

Zhang, Liu, Wang, Xie, Sheng, Zhang, Yuan, Han, Weng: "Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus)." in: Scientific reports, Vol. 7, Issue 1, pp. 16627, (2018) (PubMed).

Zheng, Wang, Zou, Jing, Yang, Li, Wang: "Ginsenoside Rb1 improves cardiac function and remodeling in heart failure." in: Experimental animals, Vol. 66, Issue 3, pp. 217-228, (2017) (PubMed).

Wang, Chen, Tang, Jiang, Ji: "AMD3100 Attenuates Matrix Metalloprotease-3 and -9 Expressions and Prevents Cartilage Degradation in a Monosodium Iodo-Acetate-Induced Rat Model of Temporomandibular Osteoarthritis." in: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, Vol. 74, Issue 5, pp. 927.e1-927.e13, (2016) (PubMed).

Liu, Jiang, Zhang, Li, Li, Xie, Hu: "Pulmonary artery denervation improves pulmonary arterial hypertension induced right ventricular dysfunction by modulating the local renin-angiotensin-aldosterone system." in: BMC cardiovascular disorders, Vol. 16, Issue 1, pp. 192, (2016) (PubMed).

Liu, Huang, Wang, Song, Cen, Ji: "Anthraquinone derivative exerted hormetic effect on the apoptosis in oxygen-glucose deprivation-induced PC12 cells via ERK and Akt activated Nrf2/HO-1 signaling pathway." in: Chemico-biological interactions, Vol. 262, pp. 1-11, (2016) (PubMed).

Chen, Shi, Li, Deng, Zhang: "Downregulation of MACC1 expression enhances cisplatin sensitivity in SKOV-3/DDP cells." in: Genetics and molecular research : GMR, Vol. 14, Issue 4, pp. 17134-44, (2016) (PubMed).

Geng, Zhu, Xie, Li, Wu, Liang, Xie, Zhu, Huang, Zhu, Wu, Zhong: "Medium-chain triglyceride ameliorates insulin resistance and inflammation in high fat diet-induced obese mice." in: European journal of nutrition, (2015) (PubMed).

Chen, Bao, Chen, Burton, Gong, Gu, Mi, Bao: "Mycobacterium tuberculosis PE25/PPE41 protein complex induces activation and maturation of dendritic cells and drives Th2-biased immune responses." in: Medical microbiology and immunology, (2015) (PubMed).

Xing, He, Yu, Hou, Chen: "Increased expression of receptor for advanced glycation end-products worsens focal brain ischemia in diabetic rats." in: Neural regeneration research, Vol. 7, Issue 13, pp. 1000-5, (2015) (PubMed).

Rosenzweig, Ou, Quinn: "P38 mitogen-activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture." in: Journal of cellular and molecular medicine, Vol. 17, Issue 4, pp. 508-17, (2013) (PubMed).

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: Erk1/2

Sub Type: Cocktail

Background:

Synonyms: ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, MAPK3

Background: p44/42 MAP Kinase(Thr202), ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

Gene ID: 5595

UniProt: P27361