PRKAB1 antibody (pSer108)

Catalog No. ABIN683053

This Rabbit Polyclonal antibody specifically detects PRKAB1 in ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for PRKAB1 antibody (pSer108) (ABIN683053)

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAB1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRKAB1 Antibody

Binding Specificity: pSer108

Cross-Reactivity: Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse AMPK beta 1 around the phosphorylation site of Ser108

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PRKAB1

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Alternative Name: AMPK beta 1

Background:

Synonyms: PRKAB1phospho S108, PRKAB1S108, AMPK beta 1Ser108, p-AMPK beta 1Ser108, p-AMPK beta 1S108, 5 AMP activated protein kinase subunit beta 1, AMPK, AMPK beta 1 chain, AMPKb, HAMPKb, PRKAB1, 5'-AMP-activated protein kinase subunit beta-1, AMP-activated protein kinase beta subunit, protein kinase, AMP-activated, noncatalytic, beta-1, AMPK beta -1 chain, 5'-AMP-activated protein kinase beta-1 subunit, AMPKb, AMPK subunit beta-1, AAKB1_RAT.

Background: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008].

Gene ID: 19079

Pathways: AMPK Signaling, Warburg Effect