CD33 antibody (AA 101-200)

Catalog No. ABIN687607

This Rabbit Polyclonal antibody specifically detects CD33 in FACS, WB, ELISA, IHC (fro), ICC, IF (p), IF (cc) and IHC (p). It exhibits reactivity toward Human and Mouse and has been mentioned in 5+ publications.

Quick Overview for CD33 antibody (AA 101-200) (ABIN687607)

Target: CD33

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD33 Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD33

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD33 antibody (ABIN687607)

Shan, Bu, Zhang, Zhang, Ding, Chang, Dai, Shen, Ma: "LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo." in: The international journal of biochemistry & cell biology, Vol. 61C, pp. 20-28, (2015) (PubMed).

Iwami, Brinkman, Bromberg: "Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics." in: Transplantation, Vol. 99, Issue 4, pp. 668-77, (2015) (PubMed).

Jiang, Li, Shi, Wu, An, Yang, Xu: "Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cells." in: Cell death & disease, Vol. 5, pp. e1270, (2015) (PubMed).

Shi, Jiang, Li, Shan, Li, An, Yang, Xu: "Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo." in: Journal of hematology & oncology, Vol. 6, Issue 1, pp. 7, (2013) (PubMed).

Target Details for CD33

Target: CD33

Alternative Name: CD33

Background:

Synonyms: gp67, Siglec-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, CD33, Siglec3

Background: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid (By similarity). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Gene ID: 12489

UniProt: Q63994