BrdU antibody (CF®488A)

Catalog No. ABIN6933666

The Mouse Monoclonal anti-BrdU antibody has been validated for FACS, IF and T. It is suitable to detect BrdU in samples from .

Quick Overview for BrdU antibody (CF®488A) (ABIN6933666)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: BRD469

Product Details anti-BrdU Antibody (CF®488A)

Specificity: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Purification: Purified by Protein A/G

Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: Bromodeoxyuridine (BrdU) (Proliferation Marker)

Target Type: Chemical

Molecular Weight: 307Da