CD31 antibody (PE)
Quick Overview for CD31 antibody (PE) (ABIN6934746)
Target
See all CD31 (PECAM1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
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No Cross-Reactivity
- Pig (Porcine), Rat (Rattus)
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Purification
- Purified by Protein A/G
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Immunogen
- Membrane preparation of a spleen from a patient with hairy cell leukemia
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Isotype
- IgG1 kappa
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Application Notes
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Positive Control: Jurkat, CCRF-CEM or K-562 cells. Tonsil, Angiosarcoma.
Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100 for 30 minutes at RT),Optimal dilution for a specific application should be determined.
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Restrictions
- For Research Use only
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Concentration
- 100 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA and 0.05 % azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Expiry Date
- 24 months
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Alternative Name
- PECAM1
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Molecular Weight
- ~100kDa (endothelium) and ~130kDa (platelets)'
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Gene ID
- 5175
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UniProt
- P16284
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Pathways
- Regulation of Actin Filament Polymerization
Target
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