CD63 antibody (PE)

Catalog No. ABIN6935956

This anti-CD63 antibody is a Mouse Monoclonal antibody detecting CD63 in FACS, IF and T. Suitable for Human and Mouse.

Quick Overview for CD63 antibody (PE) (ABIN6935956)

Target: CD63

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: NKI-C3

Product Details anti-CD63 Antibody (PE)

Specificity: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Purification: Purified by Protein A/G

Immunogen: Smooth plasma membrane fraction of MeWo cells

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100 for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD63

Target: CD63

Alternative Name: CD63

Molecular Weight: 26kDa (core protein), 30-60kDa (glycosylated)

Gene ID: 967

UniProt: P08962