CD28 antibody

Catalog No. ABIN6936660

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS, IF and Func. It is suitable to detect CD28 in samples from Human.

Quick Overview for CD28 antibody (ABIN6936660)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: 204-12

Product Details anti-CD28 Antibody

Specificity: Recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Cross-Reactivity (Details): Human. Pig. Cow. Sheep. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leucocytes

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL) Functional Studies (Order Ab without BSA & Azide), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Human lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: T-cell-specific surface glycoprotein CD28, Tp44,CD28
Cellular localisation: Cell Surface

Molecular Weight: 44kDa (monomer), 88kDa (dimer)

Gene ID: 940, 591629

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response