Elastin antibody

Catalog No. ABIN6937143

The Mouse Monoclonal anti-Elastin antibody has been validated for ELISA, Coat and IHC (f). It is suitable to detect Elastin in samples from Human.

Quick Overview for Elastin antibody (ABIN6937143)

Target: Elastin (ELN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastin antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ELN-1981

Product Details anti-Elastin Antibody

Specificity: Elastin is a polymeric protein and is the main component of the extracellular matrix of arteries.It is synthesized and secreted as a soluble, single-chain protein (tropoelastin), which undergoes a number of post-translational modifications prior to its organization into an elastic fiber in the extracellular space. Elastin performs a regulatory function during arterial development by controlling proliferation of smooth muscle and stabilizing arterial structure. It imparts elasticityto the connective tissue. During aging, the elasticity of connective tissue is reduced because of the cross-linking of collagenous fibers with elastin. The abnormal accumulation of elastic tissue in blood vessels is found in atherosclerosis and hypertension.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Elastin protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549 or U-2197 cells. Liver, Kidney, Lung or Intestine.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: ELN

Background: Elastin, ELN, SVAS, Tropoelastin, WBS, WS,Elastin (ELN) (Marker of Arterial Stiffness and Atherosclerosis)
Cellular localisation: Secreted and Extracellular Space

Molecular Weight: 70kDa

Gene ID: 2006, 647061

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization