FAF1 antibody
Quick Overview for FAF1 antibody (ABIN6937211)
Target
See all FAF1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved 'death domain' and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-RI interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-RI-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-RI via their DDH regions, while RIP associates exclusively with FAS. An additional FAS interacting protein designated FAF1, for FAS-associated protein factor-1, binds with the cytoplasmic tail of wildtype but not LPR mutant FAS. When overexpressed in cells, FAF1 enhances the efficiency of FAS-mediated apoptosis. In contrast to TRADD, FADD and RIP, FAF1 lacks a DDH and cannot induce apoptosis independently of FAS activation.
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Cross-Reactivity (Details)
- Human.
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Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogen
- Recombinant human full-length FAF1 protein
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Isotype
- IgG2b kappa
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Application Notes
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HEK293, MCF-7, HeLa whole cell lysate. Human testis, prostate, breast carcinoma or colon carcinoma.
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Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Preservative
- Azide free
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Storage
- -20 °C,-80 °C
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Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Expiry Date
- 24 months
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- FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))
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Alternative Name
- FAF1
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Background
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CGI-03, FAS-associated factor 1, HFAF1s, UBX domain-containing protein 12, UBX domain-containing protein 3A,Fas (TNFRSF6) associated factor 1
Cellular localisation: Nucleus. -
Molecular Weight
- 75-80kDa
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Gene ID
- 11124, 530402
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UniProt
- Q9UNN5
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Pathways
- Maintenance of Protein Location
Target
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