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FAF1 antibody

This Mouse Monoclonal antibody specifically detects FAF1 in WB and IHC (f). It exhibits reactivity toward Human.
Catalog No. ABIN6937211

Quick Overview for FAF1 antibody (ABIN6937211)

Target

See all FAF1 Antibodies
FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

Reactivity

  • 74
  • 30
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  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
Human

Host

  • 52
  • 22
Mouse

Clonality

  • 54
  • 20
Monoclonal

Conjugate

  • 39
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This FAF1 antibody is un-conjugated

Application

  • 61
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  • 16
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  • 8
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  • 5
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  • 1
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Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

CPTC-FAF1-2
  • Specificity

    In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved 'death domain' and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-RI interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-RI-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-RI via their DDH regions, while RIP associates exclusively with FAS. An additional FAS interacting protein designated FAF1, for FAS-associated protein factor-1, binds with the cytoplasmic tail of wildtype but not LPR mutant FAS. When overexpressed in cells, FAF1 enhances the efficiency of FAS-mediated apoptosis. In contrast to TRADD, FADD and RIP, FAF1 lacks a DDH and cannot induce apoptosis independently of FAS activation.

    Cross-Reactivity (Details)

    Human.

    Purification

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogen

    Recombinant human full-length FAF1 protein

    Isotype

    IgG2b kappa
  • Application Notes

    Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HEK293, MCF-7, HeLa whole cell lysate. Human testis, prostate, breast carcinoma or colon carcinoma.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Preservative

    Azide free

    Storage

    -20 °C,-80 °C

    Storage Comment

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Expiry Date

    24 months
  • Target

    FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

    Alternative Name

    FAF1

    Background

    CGI-03, FAS-associated factor 1, HFAF1s, UBX domain-containing protein 12, UBX domain-containing protein 3A,Fas (TNFRSF6) associated factor 1
    Cellular localisation: Nucleus.

    Molecular Weight

    75-80kDa

    Gene ID

    11124, 530402

    UniProt

    Q9UNN5

    Pathways

    Maintenance of Protein Location
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