GAD antibody (AA 72-135)

Catalog No. ABIN6937291

This anti-GAD antibody is a Mouse Monoclonal antibody detecting GAD in WB, ELISA, Coat and IHC (f). Suitable for Human.

Quick Overview for GAD antibody (AA 72-135) (ABIN6937291)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: GAD1-2391

Product Details anti-GAD Antibody

Binding Specificity: AA 72-135

Specificity: This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K-562 or HEK293 cells. Pancreas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1

Background: 67 kDa glutamic acid decarboxylase, CPSQ1, DCE1, GAD67, GAD1, Glutamate decarboxylase 1, SCP,GAD1 / GAD67 (GABAergic Neuronal Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 67kDa

Gene ID: 2571, 420036

UniProt: Q99259