GJB1 antibody

Catalog No. ABIN6937322

This Mouse Monoclonal antibody specifically detects GJB1 in WB, FACS, IF and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for GJB1 antibody (ABIN6937322)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M12-13

Product Details anti-GJB1 Antibody

Specificity: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Rat junctional complexes

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Frozen) (1-2 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Liver, Kidney, Stomach or Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1

Background: Charcot Marie Tooth neuropathy X linked, CMTX, CMTX1, Connexin-32, Cx32, GAP junction 28 kDa liver protein, Gap junction beta-1 protein, Gap junction protein beta 1 32kD, GJB1,Connexin 32 (Gap Junction Protein)
Cellular localisation: Cytoplasmic and Cell Surface

Molecular Weight: 27-32kDa

Gene ID: 2705, 333303

UniProt: P08034

Pathways: Cell-Cell Junction Organization