GJB1 antibody

Catalog No. ABIN6937323

The Mouse Monoclonal anti-GJB1 antibody has been validated for IHC (f). It is suitable to detect GJB1 in samples from Human.

Quick Overview for GJB1 antibody (ABIN6937323)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: GJB1-1753

Product Details anti-GJB1 Antibody

Specificity: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human GJB1 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Liver, Kidney, Stomach or Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1

Background: Charcot Marie Tooth neuropathy X linked, CMTX, CMTX1, Connexin-32, Cx32, GAP junction 28 kDa liver protein, Gap junction beta-1 protein, Gap junction protein beta 1 32kD, GJB1,Connexin 32 (Gap Junction Protein)
Cellular localisation: Cytoplasmic and Cell Surface

Molecular Weight: 27-32kDa

Gene ID: 2705, 333303

UniProt: P08034

Pathways: Cell-Cell Junction Organization