Myeloid Cell Marker (Macrophage / Granulocyte Marker) antibody

Catalog No. ABIN6937988

This Mouse Monoclonal antibody specifically detects in FACS, IF, IHC, StM, IHC (p) and ISt. It exhibits reactivity toward Human.

Quick Overview for Myeloid Cell Marker (Macrophage / Granulocyte Marker) antibody (ABIN6937988)

Target: Myeloid Cell Marker (Macrophage / Granulocyte Marker)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunostaining (ISt)

Clone: BM-1

Product Details

Specificity: Recognizes 183 kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.

Purification: Purified by Protein A/G

Immunogen: Human peripheral blood mononuclear cells

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HL60 cells. Bone marrow, lymph node or tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Myeloid Cell Marker (Macrophage / Granulocyte Marker)

Molecular Weight: 183kDa