Myofibroblast Marker antibody
Reactivity: Human
IHC, StM, WB
Host: Mouse
Monoclonal
PR 2D3
unconjugated
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- Target
- Myofibroblast Marker
- Reactivity
- Human
-
Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
- Specificity
- Myofibroblasts are a unique group of smooth muscle-like fibroblasts that play an important role in oncogenesis, inflammation, repair, wound contraction and fibrosis. Like smooth muscle (SM) cells, myofibroblasts contain microfilament bundles and express -SM Actin, the Actin isoform that is present in myoepithelial cells and SM cells and especially abundant in vascular SM cells. Myofibroblasts secrete inflammatory and anti-inflammatory cytokines, chemokines, growth factors and lipid and gaseous inflammatory mediators, as well as extracellular matrix proteins and proteases in most organs and tissues. Besides being temporarily present following tissue injuries and fibrocontractive diseases, myofibroblasts are also present under normal conditions in regions such as the skin, pulmonary septa and periodontal ligaments. Stem cell factor and platelet-derived growth factor (PDGF) are two secreted proteins responsible for differentiating myofibroblasts from embryological stem cells. PR 2D3 reacts with a cell membrane component of cells in the pericrypt sheath, with smooth muscle cells and myofibroblasts. This antibody is considered to be the gold standard for the identification of myofibroblasts.
- Purification
- Purified by Protein A/G
- Immunogen
- Crude homogenate of normal human colorectal mucosa
- Clone
- PR 2D3
- Isotype
- IgG1 kappa
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anti-Myofibroblast Marker antibody
Reactivity: Human IHC, StM, WB Host: Mouse Monoclonal PR 2D3 unconjugated
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- Application Notes
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Positive Control: Myofibroblasts.
Known Application: Western Blot (0.5-1.0 μg/mL) Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Buffer
- 10 mM PBS without BSA and without Azide.
- Preservative
- Azide free
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- Expiry Date
- 24 months
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- Target
- Myofibroblast Marker
- Molecular Weight
- 28kDa
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