CD73 antibody

Catalog No. ABIN6938090

This Mouse Monoclonal antibody specifically detects CD73 in WB, FACS, ELISA, IF, Coat and IHC (f). It exhibits reactivity toward Human.

Quick Overview for CD73 antibody (ABIN6938090)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NT5E-2646

Product Details anti-CD73 Antibody

Specificity: CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human NT5E protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells in 0.1ml),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431, U87MG or PC-3 cells. Tonsil, Prostate or Lung Carcinoma

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: NT5E

Background: 5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto (CD73); E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase,CD73 (Immuno-Oncology Target)
Cellular localisation: Cell surface

Molecular Weight: 71kDa

Gene ID: 4907, 153952

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process