PAX8 antibody (AA 60-261)

Catalog No. ABIN6938126

This Mouse Monoclonal antibody specifically detects PAX8 in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for PAX8 antibody (AA 60-261) (ABIN6938126)

Target: PAX8 (Paired Box 8 (PAX8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PAX8-1491

Product Details anti-PAX8 Antibody

Binding Specificity: AA 60-261

Specificity: Recognizes a protein of 62 kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 60-261) of human PAX8 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells. Renal Cell Carcinoma (RCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8

Background: Paired box 8, Paired box gene 8, paired box homeotic gene 8, Paired box protein Pax-8, Paired domain gene 8, PAX8,PAX8 (Renal Cell Marker)
Cellular localisation: Nuclear

Molecular Weight: 62kDa

Gene ID: 7849, 469728

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation