CD31 antibody

Catalog No. ABIN6938151

The Rabbit Polyclonal anti-CD31 antibody has been validated for FACS, WB and IHC (f). It is suitable to detect CD31 in samples from Human.

Quick Overview for CD31 antibody (ABIN6938151)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD31 Antibody

Specificity: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 antibody reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human recombinant CD31 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM1

Background: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell surface and cytoplasmic

Molecular Weight: ~100kDa (endothelium) and ~130kDa (platelets)

Gene ID: 5175, 376675, 514412

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization