Recombinant SERPINA1 antibody

Catalog No. ABIN6938376

Product Details anti-SERPINA1 Antibody

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SERPINA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: It recognizes a protein of 54 kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment

Clone: AAT-3167R

Isotype: IgG

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, A549 or HepG2 cells. Tonsil, hepatocellular carcinoma or histiocytoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SERPINA1

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Alternative Name: SERPINA1 (SERPINA1 Products)

Background: A1AT, Alpha-1 protease inhibitor, Alpha-1-antiproteinase (Alpha1AT), Dom1, Serine (or cysteine) proteinase inhibitor clade A member 1, Serpin peptidase inhibitor clade A member 1, Short peptide from AAT (SPAAT), Spi1-1,Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 46kDa

Gene ID: 5265, 525557

UniProt: P01009