Recombinant TAG-72 / CA72.4 antibody

Catalog No. ABIN6938509

This anti- antibody is a Mouse Monoclonal antibody detecting in IHC and StM. Suitable for Human, Dog, Cow, Rat and Hamster.

Quick Overview for Recombinant TAG-72 / CA72.4 antibody (ABIN6938509)

Target: TAG-72 / CA72.4

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog, Cow, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RB72-3

Product Details

Specificity: Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Purification: Purified by Protein A/G

Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat cells, Breast or lung carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: TAG-72 / CA72.4

Alternative Name: TAG-72 / CA72.4 (Tumor-Associated Glycoprotein)

Molecular Weight: 220kDa

Gene ID: 182875