TNFSF9 antibody

Catalog No. ABIN6938631

The Mouse Monoclonal anti-TNFSF9 antibody is suitable to detect TNFSF9 in samples from Human. It has been validated for FACS and IHC (f).

Quick Overview for TNFSF9 antibody (ABIN6938631)

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFSF9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CD137L-1547

Product Details anti-TNFSF9 Antibody

Specificity: TNFSF9 / 4-1BBL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9 / 4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9 / 4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. It has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine is required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CD137L protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells in 0.1ml), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Raji, 293T, HEK293 cells. Kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TNFSF9

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: TNFSF9

Background: 4-1BB ligand, 4-1BBL, CD137L, ILA ligand (TNF related), Ly63l, Tumor necrosis factor ligand 5A (TNLG5A), Tumor necrosis factor ligand superfamily member 9 (TNFSF9),CD137L / 4-1BBL / TNFSF9
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 27kDa

Gene ID: 8744, 1524

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints