YBX1 antibody

Catalog No. ABIN6938790

The Mouse Monoclonal anti-YBX1 antibody has been validated for IHC (f). It is suitable to detect YBX1 in samples from Human.

Quick Overview for YBX1 antibody (ABIN6938790)

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This YBX1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: YBX1-2430

Product Details anti-YBX1 Antibody

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Y-box binding protein-1 (YBX1) is the prototypic member of the cold shock protein family that fulfills numerous cellular functions. In the nucleus, YBX1 protein orchestrates transcription of proliferation-related genes, whereas in the cytoplasm it associates with mRNA and directs translation. In human tumor entities, such as breast, lung and prostate cancer, cellular YBX1 expression indicates poor clinical outcome, suggesting that YBX1 is an attractive marker to predict patients' prognosis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length YBX1 protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HEK293 or PC12 cells. Prostate, Breast or Lung Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: YBX1

Background: BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1,YBX1 / Y-box Binding Protein 1 / YB-1 (Tumor Biomarker)
Cellular localisation: Cytoplasmic and Nuclear

Molecular Weight: 36kDa

Gene ID: 4904, 473583

UniProt: P67809

Pathways: Regulation of Muscle Cell Differentiation