CYP1A1/2 antibody

Catalog No. ABIN6939223

The Mouse Monoclonal anti-CYP1A1/2 antibody has been validated for WB, IF, IHC and StM. It is suitable to detect CYP1A1/2 in samples from Human, Rat, Mouse and Primate.

Quick Overview for CYP1A1/2 antibody (ABIN6939223)

Target: CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))

Reactivity: Human, Rat, Mouse, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYP1A1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: MC1

Product Details anti-CYP1A1/2 Antibody

Purification: Purified by Protein A/G

Immunogen: 3-methylcholanthrene induced rat cytochrome P450 protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Liver or Cardiac muscle.

Known Application: Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CYP1A1/2

Target: CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))

Alternative Name: CYP1A1 / CYP1A2

Background: Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies, the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.

Molecular Weight: 58kDa

Gene ID: 1543, 1544

UniProt: P04798, P05177