alpha Fetoprotein antibody

Catalog No. ABIN6939236

This Mouse Monoclonal antibody specifically detects alpha Fetoprotein in IHC and StM. It exhibits reactivity toward Human, Pig, Dog and Monkey.

Quick Overview for alpha Fetoprotein antibody (ABIN6939236)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Pig, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: C3

Product Details anti-alpha Fetoprotein Antibody

Specificity: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

No Cross-Reactivity: Cow (Bovine), Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Alpha feto protein (AFP) Purified from serum of a hepatoma patient

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Molecular Weight: 70kDa

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process