TAG-72 / CA72.4 antibody

Catalog No. ABIN6939252

This Mouse Monoclonal antibody specifically detects in FACS, IF, IHC and StM. It exhibits reactivity toward Human, Dog, Cow, Rat and Hamster.

Quick Overview for TAG-72 / CA72.4 antibody (ABIN6939252)

Target: TAG-72 / CA72.4

Reactivity: Human, Dog, Cow, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: B72-3

Product Details

Specificity: Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Purification: Purified by Protein A/G

Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat cells, Breast or lung carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: TAG-72 / CA72.4

Alternative Name: TAG-72 / CA72.4 (Tumor-Associated Glycoprotein)

Molecular Weight: 220kDa

Gene ID: 182875