Recombinant DC-SIGN/CD209 antibody

Catalog No. ABIN6939612

The Rabbit Monoclonal anti-DC-SIGN/CD209 antibody has been validated for WB, FACS, IHC, IF, ISt and StM. It is suitable to detect DC-SIGN/CD209 in samples from Human.

Quick Overview for Recombinant DC-SIGN/CD209 antibody (ABIN6939612)

Target: DC-SIGN/CD209 (CD209) (CD209)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DC-SIGN/CD209 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Staining Methods (StM)

Clone: C209-2749R

Product Details anti-DC-SIGN/CD209 Antibody

Specificity: DC-SIGN is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human CD209 protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: THP-1 or HeLa cells (IF/FACS). Human liver or spleen lysate (WB). Human small intestine (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for DC-SIGN/CD209

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: CD209

Molecular Weight: 45kDa

Gene ID: 30835

UniProt: Q9NNX6