KRT4 antibody (AA 181-292)

Catalog No. ABIN6939863

The Mouse Monoclonal anti-KRT4 antibody has been validated for IHC, FACS, IF and StM. It is suitable to detect KRT4 in samples from Human.

Quick Overview for KRT4 antibody (AA 181-292) (ABIN6939863)

Target: KRT4 (Keratin 4 (KRT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: KRT4-2804

Product Details anti-KRT4 Antibody

Binding Specificity: AA 181-292

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa, A549 or A431 cells. Skin, Cervix or Esophagus.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT4

Target: KRT4 (Keratin 4 (KRT4))

Alternative Name: KRT4

Background: Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.

Molecular Weight: 67kDa

Gene ID: 3851

UniProt: P19013