KRT16 antibody (C-Term)

Catalog No. ABIN6939919

This anti-KRT16 antibody is a Mouse Monoclonal antibody detecting KRT16 in WB, IF, FACS, StM and IHC (fro). Suitable for Human.

Quick Overview for KRT16 antibody (C-Term) (ABIN6939919)

Target: KRT16 (Keratin 16 (KRT16))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT16 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SPM264

Product Details anti-KRT16 Antibody

Binding Specificity: C-Term

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment from the C-terminal of human Cytokeratin 16

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Tonsil, Skin. Bladder or Cervix.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Frozen & formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT16

Target: KRT16 (Keratin 16 (KRT16))

Alternative Name: KRT16

Background: Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8). The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.

Molecular Weight: 48kDa

Gene ID: 3868

UniProt: P08779