Myoglobin antibody

Catalog No. ABIN6940004

This Mouse Monoclonal antibody specifically detects Myoglobin in IHC and StM. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Myoglobin antibody (ABIN6940004)

Target: Myoglobin (MB)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myoglobin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: MB-2105

Product Details anti-Myoglobin Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length Myoglobin (MB) protein.

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: SK-BR3 or T47D cells. Skeletal or Cardiac Muscle.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Myoglobin

Target: Myoglobin (MB)

Alternative Name: Myoglobin (Muscle Cell Marker)

Background: Myoglobin is a cytosolic oxygen-binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. Myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia.In combination with other striated muscle markers such as vimentin and myogenin, myoglobin is helpful in the identification of rhabdomyosarcoma and tumors with skeletal muscle differentiation. Reportedly, myoglobin is expressed on epithelial cancer cells due to changed metabolic and environmental conditions.

Molecular Weight: 17kDa

Gene ID: 4151

UniProt: P02144

Pathways: Brown Fat Cell Differentiation