S100A9 antibody

Catalog No. ABIN6940551

The Mouse Monoclonal anti-S100A9 antibody has been validated for IHC and StM. It is suitable to detect S100A9 in samples from Human.

Quick Overview for S100A9 antibody (ABIN6940551)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CAGB-426

Product Details anti-S100A9 Antibody

Specificity: Recognizes a 12-14 kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14), expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Immunogen: Recombinant human CAGB protein

Isotype: IgM kappa

Application Details

Application Notes:

Positive Control: Tonsil, lymph node or spleen.

Known Application: Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9

Molecular Weight: 14kDa

Gene ID: 6280

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins